Excellent stability and performance could be achieved under a wide range of LC conditions with Shim-pack Scepter LC columns, which are the next generation organic silica hybrid based columns. With different chemistry characteristics, Shim-pack Scepter columns are effective for method development/scouting with suitability for use in a wide variety of applications. With different particle sizes (1.9 μm, 3 μm, 5 μm) and different column dimensions, Shim-pack Scepter LC columns are fully scalable between UHPLC, HPLC and preparative LC making method transfer seamless between different laboratory instrumentation.
With excellent stability under a wide range of LC conditions, Shim-pack Scepter LC columns are effective for method scouting combining mobile phase pH and organic modifier.
【 Comparison of Chromatograms using Gradient condition with Acetonitrile 】
| C18 | HD-C18 | C8 | Phenyl | PFPP | |
|---|---|---|---|---|---|
| pH 2.9 |  |
 |
 |
 |
 |
| pH 6.1 |  |
 |
 |
 |
 |
| pH 9.9 |  |
 |
 |
 |
Outside of
recommended pH
|
Analytes
1. Saccharin (pKa = 2.2)
2. Dextromethorphan (pKa = 8.3)
3. Amitriptyline (pKa = 9.4)
4. N-Butyl paraben (pKa = 8.3)
5. Ibupurofen (pKa = 4.4)
