For MultiNA
- DNA-500 Reagent KitThe results of analysis of the target region for the 10 types of genes related to food poisoning based on the
procedure of Fig. 1 are shown in Fig. 2.
All of the food poisoning-related genes and the targeted regions were detected.
The results of analysis using the MCE-202 MultiNA were obtained as electrophoretic images and electropherograms. The estimated size and concentration values for the amplification products were calculated from the calibration curve for the standard sample (ladder) and expressed as numeric values, allowing simple and reliable evaluation of the target amplification products.
Fig. 1 Experimental Procedure of Food Poisoning-Related Genes
Fig. 2 Analytical Results of Food Poisoning-Related Genes
DNA and RNA samples are separated by size by the electrophoresis system using a microchip so that the size of nucleic acid (DNA/RNA) samples is verified and approximately quantitated. The microchip achieves an electrophoresis system capable of speedily conducting electrophoresis separation, and a fluorescent detector ensures that analysis is performed to high sensitivity and, moreover, fully automatically.