Standard solutions (DMSO solutions) prepared within the range of 2.5 to 10 μg/L of NDMA and NDEA, respectively, were measured with the HS-20 and the GCMS-QP2020 NX, to create chromatograms and calibration curves.
Favorable linearity was obtained with a contribution ratio (R2) of 0.999 for both components, well within the above-mentioned concentration range.
Utilizing the HS-20 and the GCMS-QP2020 NX, standard solutions (DMSO solutions) prepared with 5.0 μg/L of NDMA and NDEA, respectively, were injected repeatedly over six cycles, and the peak area values were calculated. Favorable repeatability was obtained even in the low concentration region of 5.0 μg/L.
Valsartan bulk drug substances, Losartan bulk drug substances, and Olmesartan bulk drug substances were dissolved in DMSO at 5 % (w/v). Samples with NDMA and NDEA added to ensure final concentrations of 2.5, 5.0, and 10 μg/L were then subjected to additive recovery tests utilizing the HS-20 and the GCMS-QP2020 NX. Favorable results were obtained, with recovery rates in the range of 88 to 114 %.
Conditions
Standard solutions (DMSO solutions) prepared with 1.0 to 50 μg/L of NDMA and NDEA, respectively, were measured with the HS-20 and the GCMS-TQ8040 NX, to create chromatograms and calibration curves.
Favorable linearity was obtained with a contribution ratio (R2) of 0.999 for both components within the above-mentioned concentration range.
Utilizing the HS-20 and the GCMS-TQ8040 NX, standard solutions (DMSO solutions) prepared with 1.0 μg/L of NDMA and NDEA, respectively, were injected repeatedly over six cycles, and the peak area values were calculated.
Favorable repeatability results lower than 10 % were obtained, even in the low concentration region of 1.0 μg/L.
Valsartan bulk drug substances, Losartan bulk drug substances, and Olmesartan bulk drug substances were dissolved in DMSO at 2 % (w/v). Samples with NDMA and NDEA added to ensure final concentrations of 2.5, 5.0, and 10 μg/L were then subjected to additive recovery tests utilizing the HS-20 and the GCMS-TQ8040 NX. Favorable results were obtained, with recovery rates in the range of 83 to 119 %.
Conditions
A GC-MS is a general-purpose analysis instrument used in a variety of fields. The GCMS-QP2020NX heightens efficiency and can be expected to contribute to improved cost performance and a better work/life balance for users. Featuring enhanced instrument functionality, analysis software, databases, and a sample introduction system, the GCMS-QP2020 will help maximize the capabilities of your laboratory.
The Shimadzu GCMS-TQ8040 is the first triple quadrupole with Smart Productivity for high efficiency sample throughput, Smart Operation for quick and easy method development, and Smart Performance for low detection limits and Scan/MRM.
These 3 smart technologies contribute to Smart MRM, and provide the most accurate, cost effective, and easy-to-use triple quadrupole GCMS you have ever imagined